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. 2001 Dec 15;108(12):1781–1788. doi: 10.1172/JCI13568

Figure 1.

Figure 1

CIS3 and STAT3 activation in synovial tissue from patients with RA and OA. (a) Immunohistochemical detection of activated STAT3 in synovial tissues. Slides with sectioned tissues of 7-mm thickness were immunostained with anti–phospho-STAT3–specific Ab’s (α-P-STAT3) and were lightly stained with hematoxylin. (b) Expression of CIS3 mRNA in RA or OA patients and in the mouse AIA or CIA model. Total RNA was extracted from three OA and RA patients’ synovial tissues or the ankle joints of mice treated with mBSA (AIA) or bovine type II collagen (CIA). For AIA, mBSA was injected on days 0, 7, and 21. For CIA, type II collagen was injected on days 0 and 21, and RNA was extracted on indicated days. Northern hybridization was performed with cDNA probes for coding regions of human and mouse CIS3 and with control probe. (c) Cell extracts from the same CIA samples were immunoblotted with anti-phosphorylated STAT3 (PY-STAT3) or anti-STAT3 Ab’s (STAT3).