Table 2.
T-cell Mediated Autoimmune Diseases & Autoantibody Variable Region Information
| Autoimmune Disease |
Auto-Ag | V chain |
Technical approach (primers) |
VH gene family bias |
CDR3 result | Sampl e (# pat.) |
Type of Cells sampled |
Isotype | Ref. |
|---|---|---|---|---|---|---|---|---|---|
| Celiac Disease | VH & VL |
Phage library display | VH4 | - | 1 | Peripheral Blood Lymphocyte & Ileum BL??? |
IgA | [7] | |
| RA | RF | VH & VL |
Human/mouse hetrohybridoma; 5' leader degenerative sequence, 6VH |
VH4-18 & VH1 | VH4 sequences CDR3 identical |
1 | Rheumatoid synovial tissues |
IgM | [8] |
| RA | RF | VH & VL |
Hybridoma 5' leader sequence degenerate |
VH1 | 9& 16 aa long | 1 | Rheumatoid synovial tissues |
IgM | [9] |
| Sjogren's Syndrome |
a-Ro-SSA, a-La-SSB, RF |
VH & VL |
- | - | VH CDR3 shortest | - | Glandular rearrangements |
- | [10] |
| Ankylosing Spondylitis |
70 PCR results from 6 VH family primers & known JH primers. DNA used. |
VH5 over- represented; VH3 normal; VH4 under-represented |
Median of 27.2 nt (∼9aa) |
1 | Rearranged synovial membrane |
[12] | |||
| PBC | PDC-E2, BCKD-E2, OGDC, ProteinX, PDC-E1a |
VH & VL |
2 Hybridoma & phage- combinatorial library; RT- PCR with random hexamers; PCR 5' VH/VL leader & FW1 and 3' Constant Region |
- | - | 5 | Regional lymph node specific for 2-OADH autoAg |
IgM & IgG |
[13] |
| PBC | 2-OADH family |
VH & VL |
5 Hybridoma clones (1 IgM & 4 IgG). RT-PCR using oligo-dT; PCR using 5' VH leader 1-6 & VL leader and 3' Constant Region. |
VH3 & VH4 families |
3 VH clones > Replacement mutations in CDR1 & CDR2; 2 CDR3 clones longer (lypoylate-specific) than 3 CDR3 clones that recognize both (lypoylate & un) confusing paragraph |
2 | Total RNA from peripheral B-cells specific for Anti- PDC |
IgM & IgG |
[14] |