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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: J Immunol. 2007 Apr 1;178(7):4528–4537. doi: 10.4049/jimmunol.178.7.4528

FIGURE 3.

FIGURE 3

Chemokine induction during acute brain abscess development is highly dependent on MyD88. MyD88 KO and WT mice (n = 4 or 5 per group) were infected with S. aureus intracerebrally as described in Materials and Methods. Animals were sacrificed at 6, 12, or 24 h following bacterial exposure, whereupon multiplex microbead array analysis was performed on abscess homogenates to allow the simultaneous quantitation of 20 distinct mediators at the protein level. Results are shown for abscess-associated MIP-2 (A), KC (B), MIP-1α (C), and MCP-1 (D) (mean ± SD) and were normalized based on the amount of total protein recovered from abscesses to correct for differences in tissue sampling size. MIP-2 levels were determined using a standard sandwich ELISA approach because this chemokine was not included in the multiplex array. Significant differences in chemokine expression between brain abscesses of MyD88 KO and WT mice are indicated by asterisks (*, p < 0.05; **, p < 0.001). Results are representative of two independent experiments.