Table 1.

Primers

Purpose	Primer	Cloning sites
Amplimers for UGT1A1 promoter repeat analysis	5′-GTCACGTGACACAGTCAAAC-3′
	5′-TTTGCTCCTGCCAGAGGTT-3′
PCR primers for ABI automated sequencing	5′-GCCAGTTCAACTGTTGTTGCC-3′
	5′-CCACTGGGATCAACAGTATCT-3′
Nested primer for ABI automated sequencing	5′-AGAAACCTAATAAAGCTCCACC-3′
PCR primers for ABI fluorescent fragment size	5′-GCTACCTTTGTGGACTGACAGC-3′
analysis	HEX-5′-GTACAACGAGGCGTCAGGTGC-3′
To amplify 227- to 233-bp promoter fragment for	5′-GTACTTGCTGTGGTACCTCCAGAAT-3′	KpnI
functional studies	5′-GGCGCCTTTGCTCCTGCTCGAGGTTC-3′	XhoI
To amplify 259- to 265-bp promoter fragment for	5′-CGATAGGTACCTGGAAGTACTTGCTGTGGTTACTCC-3′	KpnI
functional studies	5′-ATCGCAGATCTGGCGCCTTTGCTCCTGCCAGAG-3′	BglII
pGL3 vector primers for sequencing	5′-CTAGCAAAATAGGCTGTCCC-3′
	5′-CTTTATGTTTTTGGCGTCTTCCA-3′

Italicized, positions mismatched to create restriction endonuclease digestion sites for cloning; bold, overhangs with restriction endonuclease digestion sites for cloning. ABI, Applied Biosystems.