TABLE 4.
Reactivity of sera with severe acute respiratory syndrome coronavirus (SARS-CoV) and human CoV-OC43 peptides
| Case number | S2 | 43-2 | S3 | 43-3 | S4 | 43-4 | S5 | 43-5 | SARS-CoVELISA-ODBCCDC | SARS-CoVELISA NML |
|---|---|---|---|---|---|---|---|---|---|---|
| 3 | + | + | – | – | + | – | + | + | 0.060 | neg |
| 12 | + | + | – | + | + | + | – | + | 0.825 | neg |
| 13 | + | + | + | + | + | + | + | + | 0.255 | 1:800 |
| 18 | + | + | + | – | + | + | + | + | 0.243 | neg |
| 36 | + | – | + | – | + | + | – | + | 0.435 | neg |
| 44 | + | + | + | + | + | + | + | + | 0.320 | neg |
| 48 | – | – | – | – | – | – | – | + | 0.029 | neg |
| 50 | + | + | + | + | + | + | + | + | 0.065 | 1:3200 |
| For SARS nucleocapsid protein, | For OC43 nucleocapsid protein, | |||||||||
| the sequences recognized are: | the sequences recognized are: | |||||||||
| S2=GLPNNTASWFTA | 43-2=GNVVPYYSWFSG | |||||||||
| S3=KMKELSPRWYFYYLGT | 43-3=NQRQLLPRWYFYYLGT | |||||||||
| S4=SQASSRSSSRSRGNSR | 43-4=RSAPNSRSTSRTSSRA | |||||||||
| S5=SAFFGMSRIGME | 43-5=APTAGAFFFGSRLELA | |||||||||
| S3 homology with 43-3 | Abbreviations: | |||||||||
| S4 homology with 43-4 | ELISA NML: ELISA report from the | |||||||||
| S5 homology with 43-5 | National Microbiology Laboratory | |||||||||
| S2 homology with 43-2 | ELISA-OD: Optical density reading | |||||||||
| of ELISA at the British Columbia | ||||||||||
| ‘+’ indicates patient serum reactive | Centre for Disease Control (usual | |||||||||
| to the indicated peptide spot. | cut-off is 0.20) | |||||||||