Figure 4.

Radioligand binding to GPR55. Membranes prepared from cells transiently transfected with hGPR55 or vector control were incubated with 50 nM [³H]-CP55940, [³H]-SR141716 or [³H]-WIN55,212-2. Specific binding was determined by the addition of 10 μM unlabelled ligand as competitor. The bars show the specific binding (mean±s.e.m.; n=5) determined for each ligand.