Figure 6.

Mapping G-protein coupling of GPR55. (a) Basal and 1 μM O1602 stimulated GTPγS binding (% activity, mean±s.e.m.) in human GPR55-expressing membranes in the absence and presence of various concentrations of peptides equivalent to the C termini of Gα₁₃, Gα_i1/2, Gα_i3 and Gα_s. (b) Basal and 1 μM stimulated GTPγS binding (% activity, mean±s.e.m.) in human GPR55-expressing membranes in the absence and presence of various dilutions of antibodies that bind to the C termini of Gα₁₃, Gα_i1/2, Gα_i3 and Gα_s. Data were analysed using paired t-test (^**P<0.05; ^***P<0.01; n=5).