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Transfection of Gα13 into GPR55-expressing HEK293 cells leads to an increased GTPγS signal via GPR55. Membranes prepared from HEK293s cells and HEK293s-GPR55-expressing cells were transfected with transfected with either control or Gα13-containing plasmids and tested in a GTPγS with and without 1 μM O1602. Membranes containing GPR55 demonstrate a clear increase in GTPγS binding as a result of overexpression of Gα13. Data (mean±s.e.m.) were analysed using paired t-test (**P<0.05; n=5).
