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Effects of PPARα agonists on iNOS mRNA expression in J774 macrophages. Cells were incubated with LPS (10 ng ml−1) and GW7647 (30 μM) (a) or WY14643 (100 μM) (b). Total RNA was extracted at the indicated time points and iNOS mRNA was measured by RT-PCR. The results were normalized against GAPDH mRNA. Levels of iNOS mRNA are expressed relative to that induced by LPS at 6 h (set to 100%). Results are expressed as mean±s.e.m. (n=3).
