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The effect of LPS on iNOS protein expression in J774 macrophages. J774 macrophages were stimulated by LPS (10 ng ml−1). Proteins were extracted at indicated time points and iNOS protein was measured by western blot. iNOS protein levels are expressed relative to that in LPS-treated cells at 8 h (set to 100%). Actin was used as a loading control. Values expressed are mean±s.e.m. (n=3).
