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. Author manuscript; available in PMC: 2008 Apr 15.
Published in final edited form as: Exp Cell Res. 2007 Feb 3;313(7):1373–1392. doi: 10.1016/j.yexcr.2007.01.017

Fig. 1. Expression of CAR in Sertoli and germ cells.

Fig. 1

A: Results of RT-PCR using total RNA isolated from Sertoli (SC) and germ cells (GC) to assess the steady-state mRNA level of CAR. DNA size marker is on the left (bp, base pair). Each lane represents Sertoli cell or germ cell RNA extracted from a separate batch of cells. D, day. B: A single prominent band corresponding to the apparent Mr of CAR at 46 k Da was detected on the immunoblot using Sertoli cell lysate (100 μg protein), illustrating the specificity of this antibody. DF, dye-front. C: Protein extracts of Sertoli cells (from 20-day-old rats), germ cells (from 90-day-old rats) and seminiferous tubules (from 90- day-old rats) were analyzed by immunoblotting, using a rabbit anti-CAR (H-300, Santa Cruz) polyclonal antibody. The same blot was probed with β-actin to confirm equal protein loading. Protein lysate of rat brain (from 20-day-old rats) was loaded onto the same gel, serving as positive control. D, day. D: Bar graph summarizes results of three sets of immunoblots using different batches of lysates from Sertoli and germ cells and seminiferous tubules (ST). D, day. The level of CAR in germ cells was arbitrarily set at 1 against which 1-way ANOVA was performed. *, P<0.01.