Table 2.
Sources of antibodies and their working dilutions used for different experiments in this report
| Vendor | Antibody against target proteina | Animal source | Catalog No. | Lot No. | Useb | Working dilution c |
|---|---|---|---|---|---|---|
| Santa Cruz Biotechnologies (Santa Cruz,CA) | CAR | Rabbit | sc-15405 | J1304 | IP | |
| IB | 1:200 | |||||
| IF | 1:50 | |||||
| IHC | 1:100 | |||||
| CAR | Goat | sc-10313 | F0304 | IHC | 1:100 | |
| CAR | Mouse, monoclonal | sc-32795 | E2605 | IB | 1:200 | |
| Occludin | Rabbit | sc-5562 | G2803 | IB | 1:200 | |
| Jam-C | Goat | sc-23005 | L1704 | IF | 1:50 | |
| Actin | Goat | sc-1616 | J0104 | IB | 1:200 | |
| CAR | Goat | sc-10313 | F0304 | IP | ||
| α-catenin | Rabbit | sc-7894 | A2705 | IP | ||
| β-catenin | Rabbit | sc-7199 | F0204 | IP | ||
| γ-catenin | Rabbit | sc-7900 | J139 | IP | ||
| Vinculin | Rabbit | sc-5573 | I 2204 | IP | ||
| α-actinin | Goat | sc-7453 | D292 | IP | ||
| FAK | Rabbit | sc-558 | D1806 | IP | ||
| c-Src | Mouse, monoclonal | sc-8056 | F3006 | IF | 1:100 | |
|
| ||||||
| Upstate Biotechnology (Lake Placid,NY) | PI-3kinase | Rabbit | 06497 | 25006 | IP | |
| p130 cas | Rabbit | 06500 | 19950 | IP | ||
| Src,CT,clone NL19 | Rabbit, Monoclonal | 05–772 | 26812 | IP | ||
| IB | 1:1000 | |||||
|
| ||||||
| BD Biosciences (San Jose, CA) | Integrinβ1 | Mouse, monoclonal | 610468 | 53855 | IP | |
| Espin | Mouse, monoclonal | 611656 | 32291 | IF | 1:100 | |
|
| ||||||
| Zymed Laboratories Inc. (South San Francisco, CA) | N-Cadherin | Mouse, monoclonal | 33–3900 | 50393487 | IF | 1:50 |
| ZO-1 FITC conjugate | Mouse, monoclonal | 33–91111 | 60102882 | IF | 1:50 | |
| Jam-A | Rabbit | 36–1700 | 40890360 | IB | 1:200 | |
| Goat anti-mouse FITC conjugate | Goat | 81–6511 | 40397219 | IF | 1:50 | |
| Goat anti-rabbit Cy3 conjugate | Goat | 81–6115 | 51001169 | IF | 1:50 | |
|
| ||||||
| Chemicon (Temecula, CA) | Donkey anti-mouse FITC conjugate | Donkey | AP192F | 508006630 | IF | 1:100 |
| Donkey anti-rabbit Cy3 conjugate | Donkey | AP182C | 24010618 | IF | 1:100 | |
| Donkey anti-goat FITC conjugate | Donkey | AP180F | 24011222 | IF | 1:100 | |
a
All primary antibodies used in this report were polyclonal antibodiesunless otherwise specified. It is noted that all antibodies obtained commercially cross-reacted with the corresponding rat proteins.
b
IB: Immunoblotting: IF, Immunofluorescent microscopy IHC, Immunohistochemistry IP, immunoprecipitation.
c
Working dilution of primary antibodies was prepared in ~30 ml of PBS-Tris. For all IP experiments, 2 μg IgG for each antibody against its corresponding target protein was used per reaction tube.