Figure 3.

Syk, Vav, SLP-76, and pp36–38 are tyrosine phosphorylated after thymocyte stimulation with agonist and variant peptides. Thymocytes (1 × 10⁸) from F5/β₂m°/Rag-1° mice were stimulated for 5 min with YO1 cells preloaded with 10 μM of either NP68, NP34, NP4Q, a control peptide GAG, or with unpulsed YO1 cells. Syk was immunoprecipitated with an anti-Syk antibody (A) whereas Vav was immunoprecipitated with an anti-Vav-1 antibody (B). Both SLP-76 (C) and pp36–38 (D) were immunoprecipitated with a GST-Grb2 fusion protein. Data are representative of four experiments.