Figure 4.

The peptide variants induce similar kinetics of intracellular signaling, and low concentrations of antigenic peptide induce equivalent signals to high concentrations of weak agonist and antagonist peptides. (A) Thymocytes (1 × 10⁸) from F5/β₂m°/Rag-1° mice were stimulated over a period of 30 min with YO1 cells preloaded with 10 μM of either NP68, NP34, NP4Q, or GAG, or YO1 cells alone. 0*, time taken to centrifuge cells onto the monolayer of APCs (30 sec). (B) Thymocytes stimulated for 5 min with YO1 cells preloaded with various concentrations of NP68, 10 μM to 10 pM, or 10 μM NP34, NP4Q, or GAG or YO1 cells alone. ZAP-70 and associated proteins were analyzed by immunoprecipitating with an anti-ZAP-70 antibody. Data are representative of four independent experiments.