Figure 7.

Effects of anti-CD4 and anti-CD8 Ab treatments on alloimmune responses induced after retinal allotransplantation. T cells from retinal-allotransplanted (BALB/c) mice were restimulated in vitro with donor B6 irradiated splenocytes in the presence of anti-CD4 or anti-CD8 mAb’s (2 μg/ml). The frequency of IL-2– and IFN-γ–producing T cells was measured by the ELISA spot method and the results expressed as spots per million T cells. The data shown here are representative of four separate experiments. The data show the frequency of cytokine-producing spots per million T cells.