Figure 3.
Induction of IL-4 by in vivo administration of anti-CD3 or KJ1-26. (D2 × DO10)F1 and (B6 × DO10)F1 mice were injected intravenously with 4 μg of anti-CD3 or KJ1-26, and spleens were removed after 90 min. Spleen cells (5 × 106) were cultured in 96-well plates for 2 h. Supernatants were harvested, and IL-4 was measured using the CT.4S cells. (A) Induction of IL-4 in response to in vivo treatment with anti-CD3. (B) Induction of IL-4 in response to in vivo treatment with KJ1-26. (D2 × DO10)F1 mice were injected with PBS (○) or anti-CD3 or KJ1-26 (•). (B6 × DO10)F1 mice were injected with PBS (▵) or anit-CD3 or KJ1-26 (▴).