Figure 4.

NK1.1 expression and Vα usage on thymocytes and splenocytes from DO10 (H-2^b/b) and Rag-1^−/−/DO10 mice. (A) FACS analysis of NK1.1 expression on thymocytes and splenocytes. Cells were stained with PE-anti-NK1.1 and FITC-anti-CD4, biotin-KJ1-26, or biotin-anti-Vβ8 followed by Streptavidin-FITC. The proportions of whole NK1.1⁺ cells are shown in the thymus, and NK1.1⁺CD4⁺, NK1.1⁺KJ1-26⁺, and NK1.1⁺Vβ8⁺ cells are shown in the spleen. (B) Analysis of TCRα chain usage in thymocytes and splenocytes using reverse transcription-PCR. Vα13-Jα DO (DO10 TCRα), Vα14-Jα281, and Cα transcripts were amplified with specific primers as described in Materials and Methods from B6 thymocytes (lane 1), Rag-1^−/−/DO10 thymocytes (lane 2), B6 splenocytes (lane 3), and Rag-1^−/−/DO10 splenocytes (lane 4), respectively.