Figure 3.

Amplification of exons 1 and 2 by PCR. Genomic DNA from four unrelated subjects with familial isolated hypoparathyroidism was amplified using oligonucleotide primers flanking exon 1 (left panel) or exon 2 (right panel); the proband shown in Figure 1 corresponds to subject 2. The positions of molecular-weight markers are indicated on the left. DNA from subjects 1, 3, and 4 showed amplification of appropriate-sized fragments, whereas DNA from subject 2 failed to amplify a fragment corresponding to either exon 1 or exon 2.