Figure 1.

Hp(2–20)–induced monocyte chemotaxis and activation of the monocyte NADPH-oxidase. Monocyte transmigration after 90 minutes in response to different Hp(2–20) concentrations was determined using a ChemoTx multiwell chamber system. Migration was determined microscopically by counting cells in the lower compartments. (a) Hp(2–20) induced chemotaxis in human monocytes in a dose-dependent manner. Mean values ± SEM of three separate experiments. Hp(2–20) (50 μM), but not the control peptide Hp1 (50 μM), triggered superoxide anion production in monocytes (b) with kinetics similar to that induced by the formylated peptide fMLF (inset). The Hp(2–20)–induced superoxide anion release was dose-dependent within the micromolar range (c). One representative experiment out of five (b and inset), and mean values ± SEM of five separate experiments (c).