Figure 7.

Hp(2–20) triggers functional inhibition of T cells. T cell–enriched lymphocytes were treated with culture medium (control) or admixed with 25% or 50% monocytes and Hp(2–20) (50 μM), as indicated. All cells were also treated with IL-2 (100 U/ml) during overnight incubation, followed by analysis of the activation marker CD69 in gated viable (nonapoptotic) CD3ε⁺ lymphocytes by use of flow cytometry. Hp(2–20) did not alter the IL-2–induced acquisition of CD69 in T cells when incubated with lymphocytes without monocytes (not shown). Less than 3% of CD3ε⁺ T cells expressed CD69 before the addition of IL-2 or after incubation in culture medium overnight (not shown). Similar results were obtained in three separate experiments. MO, monocytes.