Figure 4 Detection of Lactobacillus DNA. PCR is shown for faecal samples from two representative non‐separation and maternal separation probiotic‐treated rat pups. Lanes: no DNA, PCR mix with no template DNA; +ve, isolated purified L rhamnosus R0011 DNA; NS, non‐separation; MS, maternally separated; NS‐P, probiotic‐treated NS; MS‐P, probiotic‐treated MS. Probiotic DNA was observed in the samples from the MS‐P group only.