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. 2007 Jul 19;56(12):1725–1735. doi: 10.1136/gut.2007.127969

graphic file with name gt127969.f1.jpg

Figure 1 Identification of β2‐microgroglubilin (β2m) negative cells in liver sections 72 hours after intraportal infusion of bone marrow derived cells (BMDCs). Liver sections from a control animal without ischaemia/reperfusion (I/R) injury (group A). (A) Typical appearance at confocal microscopy of a fluorescence labelled cell in non‐injured liver parenchyma. (B) At immunohistochemistry for β2m a small cell with ovoid nucleus, condensed chromatin, and a small rim of β2m negative cytoplasm is visible inside a liver sinusoid (arrow). Liver sections from animals with I/R injury (group B). (C) Typical appearance at confocal microscopy of a fluorescence labelled cell in injured liver parenchyma. (D) (E) (F) At immunohistochemistry, β2m negative cells with smaller nuclei than the surrounding hepatocytes are integrated within liver parenchyma; nuclear chromatin looks less condensed than in group A (arrowheads) and the cells acquire a polygonal shape with a large cytoplasm. (Original magnification: (A), (B) ×100; (C), (D), (E), (F) ×40.)