Figure 10 Alkaline methanolysis‐HPLC analysis of bilirubin pigments in bile. (A) Bile from a wild type non‐jaundiced rat. (B) Bile from a j/j (jaundiced) Gunn rat. (C) Bile from a j/j Gunn rat one month after transplantation with bone marrow derived cells (BMDCs) from wild type animals. (D) When plain methanol was substituted for alkaline methanol in the above bile sample, the peak of bilirubin monomethylesters was selectively absent from the chromatogram, confirming its derivation from bilirubin monoglucoronide (see Results). BDC, bilirubin diconjugate; BMC, bilirubin monoconjugate; HPLC, high performance liquid chromatography; IS, internal standard; UCB, unconjugated bilirubin.