Fig. 1. Northern blot showing transcription in the GAL1-10 genes.
Total RNA was isolated from late log phase cells cultured in minimal medium containing 2% glucose or 2% galactose. The RNA was resolved on a formaldehyde-agarose gel, transferred to a membrane, and hybridized with radioactive probes. The probes were generated by random primer extension, using a 2 kb GAL1-10 fragment, encompassing the shared UAS and ~ 700 bp of each of the genes, as template. ACT1 mRNA serves as an internal loading control.