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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: DNA Repair (Amst). 2007 Jul 3;6(11):1596–1606. doi: 10.1016/j.dnarep.2007.05.005

Fig. 6. Northern blot showing transcription in the GAL1-10 genes in GAL4+ and gal4 cells.

Fig. 6

Total RNA was isolated from late log phase cells cultured in minimal medium containing 2% galactose, 3% glycerol and 2% ethanol. The RNA was resolved on a formaldehyde-agarose gel, transferred to a membrane, and hybridized with radioactive probes. The probes were generated by random primer extension, using a 2 kb GAL1-10 fragment, encompassing the shared UAS and ~ 700 bp of each of the genes, as a template. 25S rRNA serves as an internal loading control.

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