Figure 1.

Poly(A) fractionation method. Schematic representation of the poly(A) fractionation protocol. Cells or total RNA are resuspended in a GTC buffer, mixed with a radiolabelled polyadenylated probe and biotinylated oligo(dT) and diluted. The oligo(dT) is then hybridized with the RNA, resulting in stacking of the oligonucleotides on longer poly(A) tails and bound to paramagnetic beads. The beads are washed with 0.5 × SSC and the RNA is eluted using decreasing salt concentrations, eluting the RNAs with short poly(A) tails first.