Figure 5.

Inhibition of the production of IL-6 or TNFα by anti-VP-1 antibody PAR3. The experimental system is the same as that in Fig. 4. SVC from RA induce or enhance IL-6 and TNFα production by bone marrow cells (BM), tonsil cells (tonsil), a macrophage cell line U937, or THP-1 when tested at 3 days after the coculture, but those from OA do not. The addition of F(ab)′2 fragments of neutralizing antibody of B19 PAR3 (14), but not that of 1F5 with irrelevant specificity causes marked decrease of IL-6 or TNFα production in the culture supernatant. ∗∗∗, P < 0.01, ∗∗, P < 0.02.