. 2007 Sep 18;35(19):6424–6438. doi: 10.1093/nar/gkm664

Table 1.

Thermodynamic stability (T_M) and osmotic stress data for self-complementary RNA 12-mers with U:G, rF:A, rF:G and A:G pairs

Sequence	Name	T^a_M (°C)	▵n_w Ethylene glycol	▵n_w Glycerol	▵n_w Acetamide
CGCUAAUUGGCG	UG	57.7 ± 0.2	18.5 ± 3.0	20.3 ± 3.4	33.1 ± 3.0
CGCFAAUUGGCG	FG	54.9 ± 0.2	14.6 ± 2.1	26.0 ± 3.3	29.2 ± 2.4
CGCGAAUUFGCG	GF	53.9 ± 0.3	13.5 ± 2.4	24.7 ± 3.7	29.6 ± 3.1
CGCFAAUUAGCG^b	FA	53.2 ± 0.3	13.2 ± 3.1	12.7 ± 3.3	23.6 ± 2.9
CGCGAAUUAGCG	GA	58.2 ± 0.2	21.2 ± 2.6	23.8 ± 3.1	Not measured
CGCAAAUUUGCG^c	AU	58.7 ± 0.6	22.8^d	18^d	43.2^d

^a4 μM RNA, 300 mM NaCl, 10 mM Na cacodylate, pH 6.5.

^bThe crystal structure of this duplex is described in ref. (41).

^cTaken from ref. (48).

^dExperimental uncertainties were not determined.