Table 2.
Crystal data, data collection parameters and structure refinement statistics
| Structure | 5′-CGCFAAUUGGCG-3′ (FG) | 5′-CGCGAAUUAGCG-3′ (GA) |
|---|---|---|
| Crystal data | ||
| Space group | P1 | C2 |
| Number of strands per asym. unit | 4 | 1 |
| Cell dimensions | ||
| a, b, c (Å) | 22.90, 32.06, 42.93 | 41.34, 34.89, 32.12 |
| α, β, γ (°) | 96.2, 83.9, 93.9 | 90.0, 129.3, 90.0 |
| Data collection | ||
| Number of reflections | 45 752 | 13 514 |
| Resolution (last shell) | 1.10 (1.14–1.10) | 1.12 (1.16–1.12) |
| Completeness (%, last shell) | 93.9 (90.7) | 96.4 (89.3) |
| Rmerge (last shell) | 0.059 (0.239) | 0.035 (0.277) |
| Refinement | ||
| Rwork/Rfree | 0.112/0.142 | 0.147/0.187 |
| Number of atoms | ||
| RNA | 1016 | 255 |
| Water | 324 | 76 |
| Ions | – | 2 Mg2+ |
| B-factors | ||
| Nucleic acid (Å2) | 9.9 | 13.4 |
| Water (Å2) | 29.0 | 29.7 |
| r.m.s.d. | ||
| Bond lengths (Å) | 0.014 | 0.014 |
| Bond angles (1···3; Å) | 0.029 | 0.031 |