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. 2007 Sep 18;35(19):6414–6423. doi: 10.1093/nar/gkm701

Figure 2.

Figure 2.

Overexpression of rpS13 in mammalian cells suppresses the expression of mRNA from a minigene containing intron 1. (A) Diagrams of vectors pECFP-S13 and pECFP-S13-int1. (B) Relative amount of mRNA coding for rpS13 (both endogenous and plasmid-derived) in HEK 293 cells. Non-transfected cells (column 1), cells transfected with pECFP-N1 (column 2), cells transfected with pECFP-S13 (accepted as 100 arbitrary units, column 3) and cells transfected with pECFP-S13-int 1 (column 4). Experiments made in triplicate; error bars are shown. For standardization of the quantity of RNA in the samples primers specific to GAPDH cDNA were used. Dispersal of amount of GAPDH cDNA in the samples that reflects total amount of RNA was ±25% from average value.