Figure 1.

Comparison of the frequency and distribution of TLS-ERG- and NEO-transduced progenitors. Following transduction of TLS-ERG and NEO, CD34⁺Lin⁻ human CB cells were plated in methylcellulose cultures as previously described (29). The transduced progenitors were selected in 1500 μg/ml G418 (A) or not (B). The frequency and distribution of myeloid (CFU-GM, CFU-G, CFU-M), erythroid (BFU-E), and myeloid/erythroid (CFU-Mix) progenitor colonies was scored after 14 days. Colony designations were confirmed by May–Grünwald–Giemsa staining of cytospin preparations. Mean frequencies and standard deviations are graphically represented from seven independent experiments performed with seven different CB samples.