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. 1998 Jul 7;95(14):8251–8256. doi: 10.1073/pnas.95.14.8251

Figure 5.

Figure 5

Ability of nuclear extracts from TG- or albumin-treated FRTL-5 cells to bind to oligonucleotides with the sequence of the TG TTF-1/Pax-8 site (Oligo C), the TSHR TTF-1 site (Oligo TSHR), or the TG TTF-2 site (Oligo K). FRTL-5 cells grown in 6H medium were washed and exposed to 10 mg/ml 19S follicular TG or BSA as in Fig. 1. After 48 hr, extracts were prepared and incubated with the oligonucleotides noted: the oligo C site of TG, which binds TTF-1 and Pax-8 (5, 10), lanes 1–3; the TTF-1 site of the TSHR that binds only TTF-1 (9–11), lanes 4–6; and the oligo K site of TG, which binds TTF-2 (13), lanes 7–9. Complexes were evaluated by electrophoretic mobility-shift assays. The left arrow denotes the TTF-1 complex; the right arrow denotes the TTF-2 complex. The lower complex in lanes 1–3 is the Pax-8 complex, which is not present in lanes 4–6 (10). The upper complex, which appears in lane 8, represents the increased binding of Sox-4, which can interact with the TTF-2 site (34).