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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2007 Aug 19;43(11):1534–1540. doi: 10.1016/j.freeradbiomed.2007.08.005

Table 3.

Incubation of MCF-10F cells with 1–30 μM of 4-OHE2 with or without 3 μM Ro41-0960 for 24 hrs at 37 °C

4-OHE2 treatment 4-OHE2 + Ro41-0960 treatment
Compounds Detected 1 μM 10 μM 25 μM 30 μM 1 μM 10 μM 25 μM 30 μM
pmol/106 cells
4-OHE1(E2) 0.10 ± 0.03 0.63 ± 0.21 1.86 ± 0.3 2.75 ± 1.36 0.60 ± 0.05 3.64 ± 0.57 4.91 ± 0.85 5.81 ± 1.02
4-OCH3E1(E2) 70 ± 5 340 ± 34 899 ± 112 1297 ± 110 0.66 ± 0.26 6.37 ± 2.17 19.2 ± 3.5 17.9 ± 2.3
4-OHE1(E2)-2-SG 0.08 ± 0.03 0.35 ± 0.04 0.54 ± 0.23 1.1 ± 0.36 0.14 ± 0.02 0.47 ± 0.13 0.59 ± 0.18 0.87 ± 0.32
4-OHE1(E2)-2-Cys 0.25 ± 0.03 0.62 ± 0.12 3.89 ± 0.49 7.9 ± 1.93 0.3 ± 0.21 1.51 ± 0.56 16.1 ± 4.7 33.3 ± 6.4
4-OHE1(E2)-1-N7Gua 0.02 ± 0.01 0.17 ± 0.05 0.84 ± 0.27 0.85 ± 0.29 0.10 ± 0.05 0.63 ± 0.09 2.71 ± 0.42 2.79 ± 0.90
4-OHE1(E2)-1-N3Ade 0.02 ± 0.01 0.21 ± 0.05 0.92 ± 0.26 1.09 ± 0.45 0.15 ± 0.08 0.74 ± 0.12 2.77 ± 1.00 3.32 ± 0.85