Figure 4.

Di-nucleotides and BzATP do not attenuate the injury induced Ca²⁺ wave. Primary corneal epithelial cells were incubated in 5 µM Fluo-3AM for 30 min and imaged in a flow through apparatus on an LSM 510 confocal. Cells were washed in HEPES buffered saline with Ca²⁺ for at least 30 s and stimulated with the indicated nucleotide and wounded. Intensity scale is shown in (a) with red indicating highest Ca²⁺ levels and blue indicating lowest Ca²⁺ levels. The horizontal white bar in (a) represents 100 µm. (a–c) Cells were washed in HEPES buffer with Ca²⁺, stimulated with 100 µM of the indicated nucleotide, and wounded. A series of images taken from a time course of a representative experiment is presented (wound shown at asterisk). (d–f) Percent change in average fluorescence for the whole field (460 µm × 460 µm) was calculated and graphed over the time course for each experiment. Images are representative of at least 10 independent experiments. Series of images are taken from Movie 1 (see online version of article at www.springeronline.com).