Figure 8.

BzATP or ATP induces ERK1/2 and JNK1 phosphorylation in rPCNs. Rat PCNs were incubated in serum-free HGGMEM for 6 h and treated with the indicated concentration (µM) of BzATP or ATP for 5 min or with 100 µM ATP or 300 µM BzATP for the indicated time period. Cells cultured in serum-free HGGMEM (−) overnight were used as the control. Then, cell lysates were prepared and (A) ERK1/2 or (B) JNK1 phosphorylation was detected by Western analysis, as described in the Materials and methods. Total ERK1/2 and actin were used as protein loading controls for (A) and (B), respectively.