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. 2005 Dec 3;1(4):349–358. doi: 10.1007/s11302-005-8076-x

Figure 2.

Figure 2

Schematic illustration of microscale reaction of NTPDases at capillary inlet. 1. Injection of a sample of 4 nl of 320 µM of ATP (substrate) in reaction buffer containing UMP (20 µM) as an internal standard in the absence or presence of test compound (potential inhibitors) (0.3 p.s.i., 5 s); 2. Injection of enzyme (0.3 p.s.i., 5 s); 3. Injection of 320 µM of ATP (substrate) in reaction buffer containing UMP (20 µM) as an internal standard in the absence or presence of test compound (0.3 p.s.i., 5 s); 4. Overlayed plugs are then allowed to stand during a predetermined period of 5 min; 5. Subsequently a −60 µA current is applied and the reaction products migrate to the detector. Electrophoresis conditions were as described in the experimental section.