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. 2005 Dec 3;1(4):349–358. doi: 10.1007/s11302-005-8076-x

Figure 3.

Figure 3

Overlay of five electropherograms after NTPDase3 on-line reaction at the capillary inlet with different concentrations of reactive blue 2 (inhibitor) added to the substrate plug. The concentration of NTPDase3 was 0.05 µg/µl of protein, ATP: 320 µM, UMP (internal standard): 40 µM. Waiting period (duration of enzymatic reaction): 5.0 min. CE conditions: Running buffer: 50 mM potassium phosphate, pH 6.5; constant current of −60 µA; detection at 210 nm, capillary cartridge temperature: 37 °C. Reactive blue 2 (RB2) concentrations: a) 20, b) 6, c) 2, d) 0.6 and e) 0.2 µM. The lowest concentration (0.2 µM) gave virtually the same electropherogram as the control without inhibitor.