Figure 2.

Suppression by PP2C is independent of the Spc1 MAPK pathway. (A) Overexpression of pmp1⁺, and ptc1⁺ or ptc3⁺, suppressed the growth defect due to the overproduction of Pck2. Wild-type cells containing pREP1-GFP-Pck2 (Pck2 OP) and either the multicopy plasmid carrying pmp1⁺, ptc1⁺, ptc2⁺, ptc3⁺, or the control vector, were grown in EMM containing 4 μM thiamine. Cells as indicated were spotted onto the plates, and they were incubated with or without 4 μM thiamine for 4 d at 27°C. (B) Overproduction of Pck2 is toxic to wild-type cells, Δwis1, or Δspc1 cells, but not to Δpmk1 cells. The cells as indicated harboring pREP1-GFP-Pck2 were spotted onto the plates, and then they were incubated as described in Figure 2A.