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. 2007 Dec;18(12):5124–5138. doi: 10.1091/mbc.E07-06-0550

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© 2007 by The American Society for Cell Biology

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Figure 3. — Identification of the domains required for the closed conformation of GIT1. (A) Schematic representation of the closed (top) and open (bottom) conformations of GIT1. Ank, ankyrin repeats; GAP, ArfGAP domain; LZ, leucine zipper. (B–D) Flag-tagged GIT1-derived constructs were coexpressed with HA-GIT1-N in COS7 cells. Lysates were immunoprecipitated with anti-Flag mAb, and filters were probed with anti-Flag and anti-HA antibodies. (E–H) Requirement of the SHD domain of GIT1 for the interaction with the N-terminal portion of GIT1. A monomeric C-terminal polypeptide of GIT1 interacts with GIT1-ΔSHD. Lysates from cells cotransfected with the indicated GIT1-derived constructs were immunoprecipitated with anti-Flag antibodies. In each panel, immunoprecipitates (IP) were blotted with anti-Flag (top filters) and anti-HA (bottom filters) antibodies. Full-length GIT1 can associate with GIT1-C2 to form dimers via the LZ domain (E), but it cannot associate with monomeric GIT1-C2-LZ (F). Monomeric GIT1-C2-LZ can interact with GIT1-ΔSHD (G). (H) The GIT1-(229-431) construct including the SHD domain interacts with GIT1-N. (I) COS7 cells coexpressing Flag-GIT1-(229-431) with either Flag-GIT1 or Flag-GIT1-ΔSHD were lysed, immunoprecipitated with pAb SI-61 and blotted to reveal the transfected polypeptides. Ly, lysates; C, controls (beads without antibody, incubated with lysate).