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. 2007 Dec;18(12):5113–5123. doi: 10.1091/mbc.E07-04-0330

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© 2007 by The American Society for Cell Biology

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Figure 5. — ESI-MS analysis of the distribution of PyrSMs and unlabeled SMs between PM vesicles and cells. Pyr₄SM, Pyr₁₂SM, and Pyr₁₄SM association with PM vesicles isolated from fibroblasts after 1-h labeling and 2-h chase is expressed as percentage of total PyrSM in PM vesicles (A) and long-chain/short-chain PyrSM ratio in PM vesicles and cells (B) (n = 6). Distribution of unlabeled SM species between cells and vesicles isolated after 1 h labeling and 2-h or 2- to 3-d chase is expressed as percentage of SM in PM vesicles (C) and long-chain/short-chain SM ratio in PM vesicles and cells (D) (n = 9–22). Error bars are SEM. **p < 0.01, ***p < 0.001.