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Examples of extra- and intracellular calcification by nanobacteria. TEM micrograph of cultured nanobacteria (bar = 200 nm) from FBS (A) and a bacterium in a kidney stone after demineralization (B; bar = 50 nm). (C) IIFS of the same kidney stone with anti-nanobacteria mAb. (D and E) von Kossa staining results of 3T6 cells exposed to SF nanobacteria for 24 hr. (F) Negative control. (×270.)
