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Inactivation of PaHsp104 affects thermotolerance. A wild-type, a ΔPaHsp104, and a ΔPaHsp104 :: pCB-PaHsp104 strain (bearing an ectopic copy of PaHsp104) were inoculated on solid medium, heat-shocked at 48°C for 2 h, and incubated for 24 h at 26°C. The procedure was repeated two more times before imaging the Petri plates.
