Table 6.
Effect of PaHsp104 deletion and over-expression on infectivity of HET-s(218–289) amyloid fibers
| Amount of HET-s(218–289) amyloid fibers |
No protein | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 2 nmol | 1 nmol | 0.5 nmol | 0.25 nmol | 120 pmol | 60 pmol | 30 pmol | 15 pmol | ||
| Recipient straina | |||||||||
| Experiment 1 | |||||||||
| wt | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 1/3 | 0/6 |
| ΔPaHSP104 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/6 |
| pGPD-PaHsp104 | 1/3 | 1/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/6 |
| Experiment 2 | |||||||||
| wt | ND | 3/3 | 3/3 | ND | 3/3 | ND | ND | 0/3 | 0/6 |
| ΔPaHSP104 | ND | 0/6 | 0/6 | ND | 0/6 | ND | ND | 0/6 | 0/6 |
| pGPD-PaHsp104 | ND | 6/6 | 3/6 | ND | 1/6 | ND | ND | 0/6 | 0/6 |
| Experiment 3 | |||||||||
| wt | ND | 6/6 | 6/6 | ND | 6/6 | ND | ND | 6/6 | 0/6 |
| ΔPaHSP104 | ND | 0/12 | 0/12 | ND | 0/12 | ND | ND | 0/12 | 0/6 |
| pGPD-PaHsp104 | ND | 8/12 | 7/12 | ND | 3/12 | ND | ND | 0/12 | 0/6 |
| Total | |||||||||
| wt | 3/3 | 12/12 | 12/12 | 3/3 | 12/12 | 3/3 | 3/3 | 7/12 | 0/18 |
| ΔPaHSP104 | 0/3 | 0/21 | 0/21 | 0/3 | 0/21 | 0/3 | 0/3 | 0/21 | 0/18 |
| pGPD-PaHsp104 | 1/3 | 15/21 | 10/21 | 0/3 | 4/21 | 0/3 | 0/3 | 0/21 | 0/18 |
a Infected strains over total tested strains.