Figure 6.

CAP recruits FLNc to cell-ECM adhesions. (A) L6 myoblasts were transiently transfected with myc-tagged wild-type CAP or the W2F mutant and costained with anti-myc (green) and anti-FLNc (red) antibodies. (B) FLNc coprecipitated with paxillin in the presence of CAP. COS-1 cells were transfected with HA-FLNc, GFP-paxillin, and either empty vector, myc-tagged WT CAP, or the W2F mutant. Cells were lysed and immunoprecipitated with anti-HA antibody, and the coprecipitated GFP-paxillin was detected by Western blot analysis.