Figure 8.

Overexpression of CAP inhibits FLNc induced cell spreading on fibronectin. COS-1 cells were transfected with GFP control, HA-FLNc, GFP-CAP, or both. Cells were then plated on fibronectin-coated plates for indicated time and photographed. A representative field of each time points was shown (A), and the percentage of spread cells was calculated from five fields (B). More than 90% of cells were transfected when examined by GFP fluorescence and HA staining of an aliquot of the cells (data not shown). Results represent one of three experiments. The data represent mean ± SE. *p <0.05, compared with GFP; ^#p <0.02, compared with HA-FLNc.