Figure 7.

NPPD disrupts filamentous actin and reverses the effects of GEF1 overexpression on the secretory pathway. Cells transfected with plasmid encoding Kal-GEF1 or its parental vector (control) were treated for 90 min with DMSO or NPPD (100 μM). Filamentous actin (A) and POMC (B) were visualized. In B GEF1 expressors are indicated by arrowheads. The inset region is shown with enhanced luminosity to show the GEF1 expressor; scale bar, 20 μm. (C) Lysates from cells treated as in A and B were extracted and probed for POMC product by immunoblotting. Enrichment of mature 16 kDa is measured relative to POMC (n = 3).