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. 2007 Dec;18(12):5060–5068. doi: 10.1091/mbc.E07-04-0327

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© 2007 by The American Society for Cell Biology

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Figure 5. — Effect of individual SP/TP mutations on tau phosphorylation at disease-associated sites. (A) Generation of individual phosphoepitopes occurs primarily by independent phosphorylation events. Fly head extracts were subject to Western immunoblot analysis by using the following antibodies: AT270 (pT175/pT181), AT8 (pS202/pT205), Tau1 (recognizes unphosphorylated epitopes between amino acids 189 and 207), TG3 (recognizes pT231/pS235), PHF1 (PS396/pS404), and a polyclonal antibody against total tau. (B) Generation of the AT100 epitope requires phosphorylation of T212, S214, and S199/T217. Equivalent amounts of fly head extracts derived from transgenic animals expressing wild type and mutant forms of tau were analyzed by Western immunoblotting by using the following antibodies: AT100 (T212/S214), pThr212, pSer214, and a polyclonal antibody specific for total tau. Driver is GMR-GAL4.