Figure 6.

Blocking phosphorylation at five SP/TP sites does not inhibit tau toxicity. (A) The tau^AP5 mutant has the following five mutations: S202A, T205A, T212A, T231A, and S235A. This manipulation disrupts reactivity with the AT8, AT100, and TG3 antibodies. (B) Tau^AP5-expressing flies have the same retinal toxicity as tau^WT flies. (C) Western blot analysis confirms that tau^AP-expressing flies are not reactive for AT8, AT100, and TG3, whereas phosphorylation at other disease-associated sites, including T181 (AT270), S214 (pSer214), and S396/S404 (PHF1) is maintained. Reactivity with the Tau1 antibody is also observed. Tau^WT and tau^AP5 expression levels are equivalent, as demonstrated by immunoblot by using a polyclonal total tau antibody. Driver is GMR-GAL4.