Fig. 6.

Effect of 5′-nucleotidase inhibitors on growth of EPEC strain E2348/69 in minimal medium supplemented with 30 μM adenosine or AMP. Minimal medium here refers to M9 salts, 2% casamino acids, and 2 mM glucose. Growth was at 37°C with 300 rpm shaking after a 1: 2,000 dilution from an overnight culture in LB medium. a, c Culture turbidity by spectrophotometric absorbance at 600 nm. b Bacterial protein content. a, b Growth-enhancing effects of AMP are blocked by α,β-methylene-ADP. c Growth-enhancing effects of AMP are blocked by zinc acetate; *significantly decreased compared to AMP alone (p < 0.05)