. 2007 May 17;3(3):233–246. doi: 10.1007/s11302-007-9056-0

Table 1.

Effect of EPEC infection on expression of RNA encoding ecto-5′-nucleotidase in T84 cells, by reverse transcription and real-time PCR

Condition	Normalized expression ratio^a
Uninfected control	1.0, by definition
+1 U/ml PI-PLC	1.23 ± 0.26
HS-infected^b	0.66 ± 0.22
E2348/69-infected^b	1.33 ± 0.21^c
UMD874 (espF)-infected^b	1.40 ± 0.19^c

^aCells were infected for 35 min, then the supernatant medium was changed to remove unattached bacteria, then the T84 cell monolayer harvested for RNA extraction 4 h after the medium change. Expression of ecto-5′-nucleotidase was normalized to that of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Data shown are the means ± SD of 5 similar experiments

^bBacteria were added with the intent of achieving a multiplicity of infection (MOI) of 100:1; actual MOIs in the 5 experiments ranged from 71 to 144

^cSignificantly increased compared to uninfected control by paired t-test (p = 0.04)